Genetic diversity of genus Brucella in Croatia and neighbouring countries
Sanja Duvnjak, Silvio Špičić, Maja Zdelar-Tuk, Ivana Račić, Željko Cvetnić
Hrvatski veterinarski institut Zagreb, Hrvatska
E-mail: sanja.duvnjak@gmail.com
Bruceloza je vrlo česta bakterijska zoonoza u svijetu, osobito u mediteranskim zemljama. Regionalni nedostatak epidemioloških istraživanja unazad par desetljeća onemogućava efikasnu kontrolu širenja bolesti. Vrlo velika genetska homogenost roda Brucella izaziva nepremostive probleme prilikom podtipiziranja mikrobiološkim i standardnim molekularnim tehnikama. Istim tehnikama određena su i tipizirana 264 izolata Brucella sp. podrijetlom iz zemalja jugoistočne Europe, pohranjeno u arhivi Hrvatskog veterinarskog instituta, Zagreb.
Proučavanje 16 lokusa koji sadrže različiti broj uzastopnih ponavljanja (MLVA-16) pokazalo se kao trenutačno najprikladnija metoda genotipizacije koja posjeduje i internacionalnu bazu genotipova. Potvrdili smo da metoda ima dijagnostički potencijal, ali ne i na razini biovara. Sojevi se grupiraju zajedno na osnovi filogenetske i geografske povezanosti otkrivajući tako regionalnu specifičnost i posebnost koje upućuju na načine širenja među životinjama.
Unijeli smo više od 50 novih genotipova u međunarodnu bazu te uveli metodu u rutinsku upotrebu. Ovi koraci bi trebali pridonijeti globalnim naporima kontrole i iskorjenjivanja bruceloze, pogotovo ako uslijede istraživanja na široj regionalnoj razini.
← BRUCELOZA U HRVATSKOJ I SUSJEDNIM ZEMLJAMA
Genetic diversity of genus Brucella in Croatia and neighbouring countries
Sanja Duvnjak, Silvio Špičić, Maja Zdelar-Tuk, Ivana Račić, Željko Cvetnić
Croatian Veterinary Institute Zagreb, Hrvatska
E-mail: sanja.duvnjak@gmail.com
Brucellosis is the very often common bacterial zoonosis in the world, particularly in Mediterranean countries. The lack of epidemiological studies in recent decades has hampered efforts to control disease spread. The extreme genetic homogeneity of Brucella sp. Isolates results in subtyping difficulties using microbiological and standard molecular techniques.
Different microbiological and molecular techniques were used to identify and type 264 isolates originating from SE Europe, present in the archive of Croatian Veterinary Institute, Zagreb. Multilocus variable-number tandem repeat analysis 16 (MLVA-16) was used for subtyping because it is currently the most appropriate method and is associated with an international genotype database. It was confirmed to have diagnostic potential, but not at the biovar level. Strains were grouped according to phylogenetic and geographic relationships, revealing both regional specificity and uniqueness and suggesting possible sources and modes of spread among animals. We have deposited more than 50 novel genotypes into the international database and introduced the method into routine use. These steps, especially if followed by further ones on wider regional scale, should contribute to global efforts to control and eradicate brucellosis.
