Relja Beck (1), Ana Beck (2), Josip Kusak (2), Željko Mihaljević (1), Snježana Lučinger (2), Tatjana Živičnjak (1), Đuro Huber (2), Andrea Gudan-Kurilj (2), Albert Marinculić (2)
(1) Hrvatski veterinarski institut u Zagrebu, (2) Veterinarski fakultet Sveučilišta u Zagrebu, E-mail: relja.beck@gmail.com
Dr. sc. Relja Beck
(Hrvatski veterinarski institut)
C ilj je bio istražiti proširenost invazije trihinelom u vukova (Canis lupus) na 17 468 km2 njihove rasprostranjenosti u Hrvatskoj. Uzorci mišićja prikupljeni su od 67 vukova u razdoblju od 1996. do 2007. i analizirani metodom umjetne probave. Ličinke u mišićima nađene su u 21 vuka (31%) i genotipizirane su metodom multiplex PCR. Trichinella britovi je bila dominirajuća vrsta potvrđena u 90% (19 vukova) dok je Trichinella spiralis utvrđena u 9% (2 vuka). Nalaz tzv. „domaće“ vrste trihinele bio je iznenađujući, jer je u tom području u divljih životinja do danas bila zabilježena jedino T. britovi. Opterećenje ličinkama u zaraženih životinja kretalo se u rasponu od 0,3 do 45,9 ličinki po gramu mišićja.
Pojavljivanje zaraženih životinja razlikovalo se po istraživanim regijama. U Gorskom kotaru učestalost je bila 20%, a stanište tog područja je dosta slično staništu u Lici gdje su skoro svi vukovi bili zaraženi. Zanimljivo je da su zabilježeni i prvi pozitivni vukovi u Dalmaciji.
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Trichinellosis in wolves from Croatia
Relja Beck, Ana Beck, Josip Kusak, Željko Mihaljević, Snježana Lučinger, Tatjana Živičnjak, Đuro Huber, Andrea Gudan, Albert Marinculić
E-mail: relja.beck@gmail.com
The aim of the present study was to investigate the prevalence of Trichinella infection in wolves (Canis lupus) in a 17,468 km2 area in Croatia. Muscle samples were collected from 67 wolves between 1996 and 2007 and analyzed by artificial digestion. Muscle larvae were detected in 21 wolves (31%) and genotyped by multiplex PCR. Trichinella britovi was the predominant species confirmed in 90% (19 wolves) while Trichinella spiralis was detected in 9% (2 wolves). The presence of the so called “domestic” Trichinella species was a surprise since, to date only T. britovi had been reported in wild animals in this region. The larval burdens in infected animals ranged from 0.3 to 45.9 larvae per gram. The prevalence of infected animals varied by geographic region.
Infected animals were found in the region of Gorski Kotar (20%) which has very similar environment to the region of Lika, where almost all wolves were found infected. Interestingly, this is the first report of infected wolves in Dalmatia.
